SynerGPCR organises GPCR assay data into a four-layer functional chain, each layer classified by its readout in the signalling cascade:
About — assay layer definitions · Bliss Synergy Index · data sources Help — how to search · reading assay chain diagrams · AI confidence scores
Assay Chain Depth and Clinical Approval Synergy
Approval rates remain below 1% for compounds assessed at any single signalling layer.
Multi-layer assay activity co-occurs with clinical approval synergistically — quantified using the Bliss Synergy Index (SI).
Full chain: L1 (Receptor Binding) + L2 (G-Protein Coupling) + L3 (β-Arrestin Recruitment) + L4 (Reporter Gene Activity).
AI-Based Chain Completion
GPCRact (an E(n)-equivariant graph neural network) predicts missing G-protein coupling and β-arrestin activities de novo, recovering approximately 79% of the experimental synergy benchmark at both signalling layers.
(a) G-Protein Coupling (L2)
(b) β-Arrestin Recruitment (L3)
GPCRact mode-of-action prediction: Balanced Accuracy = 0.875 for G-Protein Coupling (L2) and 0.834 for β-Arrestin Recruitment (L3), evaluated on held-out approved drugs.